|
Twitter
|
Facebook
|
Google+
|
VKontakte
|
LinkedIn
|
 
 
International Journal of Innovation and Scientific Research
ISSN: 2351-8014
 
 
Monday 22 July 2019

About IJISR

News

Submission

Downloads

Archives

Custom Search

Contact

Connect with IJISR

   
 
 
 

Characterization of emulsification activity of partially purified Rhamnolipids from Pseudomonas fluorescens


Volume 3, Issue 1, June 2014, Pages 88–100

 Characterization of emulsification activity of partially purified Rhamnolipids from Pseudomonas fluorescens

Jyotsna K Peter and Dhirendra Pratap Singh

Original language: English

Received 22 April 2014

Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract


In recent years natural biosurfactants have attracted attention because of their low toxicity, biodegradability, and ecological acceptability. Rhamnolipids is composed of rhamnose sugar molecule and β-hydroxyalkanoic acid. Soil isolates of Pseudomonas fluorescens was used to characterize substrate (Mustard oil, Soybean oil, Olive oil, Palm oil, Sunflower oil and Coconut oil) for highest rhamnolipid production. Highest yield of biosurfactants was obtained from Soybean oil as 7.16 g/L by P. fluorescens. The estimated value of rhamnolipids was 0.437 g/L using Soybean oil from P. fluorescens by Orcinol method. Bacterium was capable of emulsifying a wide range of vegetable oils. The emulsification activity was found stable up to 72 hours. Upon characterizing C:N ratio of higher rhamnolipid was obtained at C:N ratio of 40 (93.75%) for P. fluorescens using Soybean oil as carbon source and Ammonium chloride as nitrogen source. It was revealed that partially purified rhamnolipid of P. fluorescens showed highest emulsification at optimum temperature 4°C for Palm oil, Sunflower oil and Coconut oil (90% all), at pH 6 and pH 7 in Palm oil, Sunflower oil and Coconut oil (90% all) and for 5% NaCl concentration in palm oil (94.44%).

Author Keywords: Rhamnolipid biosurfactant, Pseudomonas fluorescens, emulsification activity.


How to Cite this Article


Jyotsna K Peter and Dhirendra Pratap Singh, “Characterization of emulsification activity of partially purified Rhamnolipids from Pseudomonas fluorescens,” International Journal of Innovation and Scientific Research, vol. 3, no. 1, pp. 88–100, June 2014.