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International Journal of Innovation and Scientific Research
ISSN: 2351-8014
 
 
Monday 23 July 2018

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Activity of Acetic Acid on Bacillus stearothermophilus and Bacillus subtilis Spores after Sublethal Heat Pretreatments


Volume 10, Issue 2, October 2014, Pages 570–575

 Activity of Acetic Acid on Bacillus stearothermophilus and Bacillus subtilis Spores after Sublethal Heat Pretreatments

James Ronald BAYOÏ, Darman Roger DJOULDE, Patrice BOGNE KAMGA, Maximilienne NYEGUE, Steve VOUNDI OLUGU, Daoudou BAKARY, and Francois-Xavier ETOA

Original language: English

Received 20 August 2014

Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract


This work has been led in view to find the influence of sublethal heat (45°C, 50°C, 60°C) on acid resistance of B. subtilis NCTC 3610 and B. stearothermophilus CNCH 5781 spores. Firstly, we have submitted Bacillus spores to 0.4% acetic acid pH 4.5 during the times of 1, 2 and 3 hours. Then another spores group were preheated at various sublethal temperatures, before be treated with acetic acid. The effect of acetic acid before and after preheat was evaluated by the culture of treated spores on agar medium and the number of colony obtained was compared with that of control culture (neither treated with heat nor acid) and control A culture (only treated with acid). We found that acetic acid was effective on the twice Bacillus spores with more effect on B. stearothermophilus CNCH 5781 spores. Furthermore we have noticed a significant increasing in percentages of recovery of colonies obtained from preheated and acid treated spores compared to those of control and control A cultures. This increase of recovery percentages could be demonstrated the manifestation of a "heat-induced acid resistance" phenomenon. Yet, this phenomenon was more accentuated for preheatings at 50 and 60°C during 3 and 2 hours, respectively for B. subtilis and B. stearothermophilus spores. This study suggest that sublethal heats could be play major role in protection of microorganisms to chemicals

Author Keywords: sublethal heats, acid resistance, spores, Bacillus.


How to Cite this Article


James Ronald BAYOÏ, Darman Roger DJOULDE, Patrice BOGNE KAMGA, Maximilienne NYEGUE, Steve VOUNDI OLUGU, Daoudou BAKARY, and Francois-Xavier ETOA, “Activity of Acetic Acid on Bacillus stearothermophilus and Bacillus subtilis Spores after Sublethal Heat Pretreatments,” International Journal of Innovation and Scientific Research, vol. 10, no. 2, pp. 570–575, October 2014.