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International Journal of Innovation and Scientific Research
ISSN: 2351-8014
 
 
Monday 23 July 2018

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Micropropagation of Vitex negundo L. - A Significant Medicinal Plant


Volume 12, Issue 2, December 2014, Pages 480–483

 Micropropagation of Vitex negundo L. - A Significant Medicinal Plant

Shamima Nasrin, Md. Shamim Hossain, Md. Ashrafuzzaman Sapon, and Monokesh Kumer Sen

Original language: English

Received 7 November 2014

Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract


Introduction: Vitex negundo L. (Nisinda locally), belongs to the family Verbenaceae, found almost everywhere in Bangladesh is a medicinal aromatic shrub. Materials and Methods: An attempt was taken to its micropropagation from field-grown explants (shoot-tip) in Murashige and Skoog medium fortified with various concentrations of phytohormones. Results: Experimentally, the best shoot induction was observed in full strength MS medium supplemented with BAP 1.0mg/L and Kin 0.5mg/L. However, 0.5mg/L IBA in half strength MS medium was enabled to induce 80% root initiation with the highest root number and longest shoot length. Well-developed roots were successfully subjected to hardening process and acclimatized. Conclusion: Regenerated plantlets were same as the natural plants and showed 80.56% survival frequency with frisky and seductive appearances without any abnormalities.

Author Keywords: Vitex negundo, micropropagation, Murashige and Skoog medium, acclimatized.


How to Cite this Article


Shamima Nasrin, Md. Shamim Hossain, Md. Ashrafuzzaman Sapon, and Monokesh Kumer Sen, “Micropropagation of Vitex negundo L. - A Significant Medicinal Plant,” International Journal of Innovation and Scientific Research, vol. 12, no. 2, pp. 480–483, December 2014.