The tomato (Lycopersicon esculentum) is one of the most important vegetable crops in the world. However, in Côte d'Ivoire, there is a general decline in yield and quality of production compared to the capacity of the cultivars). The general objective of this study is to contribute to improve the growth of tomato in nursery using organic substrate. Specifically, the germination rate and speed, stem height and number of leaves were determined for each of the formulations in order to determine the ideal substrate formulation in the nursery. The plant material was tomato seed of the variety Cobra 26. The substrates used are soil and carbonized rice husks. The technical equipment consisted of honeycombed plates, a graduated ruler and a mini sprayer. The results obtained show a significant variation of the germination speed according to the formulations. During the growth of the plants in the nursery, the average height of the stems and the average number of leaves, the color of the leaves and the sanitary state of the plants for each formulation were determined and the analysis of the results showed a significant variation of these four variables in relation to the formulations. Formulations including ¼ charred rice husk and ¾ soil gave the best results overall.